Relaxin as a diagnostic tool for pregnancy in the coyote (Canis latrans).
نویسندگان
چکیده
The diagnosis of pregnancy in the domestic dog (Canis familiaris) often employs specialized equipment, experienced staff, and the cooperation of the bitch. These procedures can be challenging when the subject is a wild canid, particularly in a field setting. In addition, reproductive hormone assays are unreliable as a diagnostic tool because the estrous profiles of pregnant and pseudopregnant canines are similar. However, research has demonstrated that the hormone relaxin can be detected in maternal blood after embryonic implantation, but remains negligible in non-pregnant females. We investigated the use of relaxin as a diagnostic marker of pregnancy in the coyote (C. latrans). A commercially available canine relaxin enzyme immunoassay (ReproCHEK) was used to test plasma collected from 124 female coyotes over four consecutive breeding seasons. Mating activities of the captive females were observed; then peripheral blood samples were collected at intervals throughout pregnancy, as well as after parturition. Results demonstrated that relaxin could be detected in the plasma of pregnant coyotes after 28 days of gestation, and in some cases as early as 23 days, while non-pregnant females and male coyotes consistently tested negative. Relaxin also remained detectable in the plasma of the majority of females tested 10-12 weeks after parturition. This qualitative assay for relaxin proved to be a reliable diagnostic tool for pregnancy in the coyote. In addition, blood sampling was relatively easy, could be accomplished with minimal handling, and did not require sedation or anesthesia.
منابع مشابه
Reproductive Biology of the Coyote (canis Latrans): Integration of Mating Behavior, Reproductive Hormones, and Vaginal Cytology
The reproductive biology of wild Canis species is often described as unique among mammals because an unusual combination of behavioral and physiological characteristics including a seasonally monestrous cycle, copulatory lock or tie, obligatory pseudopregnancy, social monogamy, and biparental care of the young. We investigated social behavior, endocrine profiles, and vaginal cytology of female ...
متن کاملTaxonomic Implications of Morphological and Genetic Differences in Northeastern Coyotes (Coywolves) (Canis latrans × C. lycaon), Western Coyotes (C. latrans), and Eastern Wolves (C. lycaon or C. lupus lycaon)
common in nature, having been documented in amphibians, insects, fish, birds, and especially within closely related plant species (Berger 1973; arnold 1992; Fritz et al. 1994; haddad et al. 1994; Parris et al. 1999; arnold et al. 1999; albert et al. 2006; Schierenbeck and Ellstrand 2009; Meyerson et al. 2010). allendorf et al. (2001) noted that hybridization is more common in fish than in other...
متن کاملValidation for use with coyotes (Canis latrans) of a commercially available enzyme-linked immunosorbent assay for Dirofilaria immitis.
Serological tests offer a potentially powerful tool for monitoring parasites in wildlife populations. However, such tests must be validated before using them with target wildlife populations. We evaluated in coyotes (Canis latrans) the performance of a commercially available serological test used to detect canine heartworm (Dirofilaria immitis) in domestic dogs. We obtained 265 coyote carcasses...
متن کاملNortheastern coyote / coywolf taxonomy and admixture : A meta - analysis
A flurry of recent papers have attempted to taxonomically characterise eastern canids, mainly grey wolves Canis lupus, eastern wolves Canis lycaon or Canis lupus lycaon and northeastern coyotes or coywolves Canis latrans, Canis latrans var. or Canis latrans x C. lycaon, in northeastern North America. In this paper, we performed a meta-analysis on northeastern coyote taxonomy by comparing result...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Animal reproduction science
دوره 101 3-4 شماره
صفحات -
تاریخ انتشار 2007